Journal: The Scientific World Journal

Article Title: In Vivo Expression of Interleukin-37 Reduces Local and Systemic Inflammation in Concanavalin A-Induced Hepatitis

doi: 10.1100/2011/968479

Figure Lengend Snippet: In vivo luciferase activity in the liver of C57/BL/6 mice after hydrodynamic tail vein injection of plasmid-DNA. C57/Bl6 mice were rapidly injected with pTarget-IL-37 (20 μ g) and pLuc plasmid-DNA (1 μ g) in 2 mL of Ringer's solution. After 48 hrs concanavalin A 200 μ g was applied into the tail vein. 24 hrs after ConA injection, luciferase expression was measured in vivo using the IVIS imaging system (a). Luciferase activity in IL-37-expressing mice (open bar) and control mice (closed bars) (b). IL-37 protein detected in liver lysate of mice injected with IL-37-pTarget plasmid mice and control mice (empty pTarget) by Western blotting (c).

Article Snippet: Immunostaining against IL-37 in formalin-fixed liver tissue was performed with a polyclonal antibody against IL-37 (anti-human IL-1F7/FIL1 zeta goat IgG R&D-Systems) and a mouse monoclonal Ab [ ].

Techniques: In Vivo, Luciferase, Activity Assay, Injection, Plasmid Preparation, Expressing, Imaging, Western Blot

Journal: The Scientific World Journal

Article Title: In Vivo Expression of Interleukin-37 Reduces Local and Systemic Inflammation in Concanavalin A-Induced Hepatitis

doi: 10.1100/2011/968479

Figure Lengend Snippet: Detection of transgene IL-37 by immunohistochemistry. 5 μ m sections of mouse livers after plasmid-DNA injection with control plasmid (empty pTarget) or IL-37-pTarget were stained with a polyclonal antibody against IL-37.

Article Snippet: Immunostaining against IL-37 in formalin-fixed liver tissue was performed with a polyclonal antibody against IL-37 (anti-human IL-1F7/FIL1 zeta goat IgG R&D-Systems) and a mouse monoclonal Ab [ ].

Techniques: Immunohistochemistry, Plasmid Preparation, Injection, Staining

Journal: The Scientific World Journal

Article Title: In Vivo Expression of Interleukin-37 Reduces Local and Systemic Inflammation in Concanavalin A-Induced Hepatitis

doi: 10.1100/2011/968479

Figure Lengend Snippet: LPS-induced serum cytokines in mice expressing IL-37. Tail vein injected C57/Bl6 mice (with or without IL-1F7b plasmid-DNA) were challenged with LPS (10 μ g) intraperintoneally ( n = 7–9). After 2 hrs the mice were sacrificed and serum cytokines were measured by a multiarray cytokine assay as described.

Article Snippet: Immunostaining against IL-37 in formalin-fixed liver tissue was performed with a polyclonal antibody against IL-37 (anti-human IL-1F7/FIL1 zeta goat IgG R&D-Systems) and a mouse monoclonal Ab [ ].

Techniques: Expressing, Injection, Plasmid Preparation, Cytokine Assay

Journal: The Scientific World Journal

Article Title: In Vivo Expression of Interleukin-37 Reduces Local and Systemic Inflammation in Concanavalin A-Induced Hepatitis

doi: 10.1100/2011/968479

Figure Lengend Snippet: Expression of serum cytokines in IL-37-expressing mice after ConA-induced hepatitis. Serum cytokines were measured by a multiarray cytokine assay 2 hrs (a) and 24 hrs (b) after ConA treatment in IL-37 expressing mice (open bars, n = 4) and control mice (closed bars, n = 3).

Article Snippet: Immunostaining against IL-37 in formalin-fixed liver tissue was performed with a polyclonal antibody against IL-37 (anti-human IL-1F7/FIL1 zeta goat IgG R&D-Systems) and a mouse monoclonal Ab [ ].

Techniques: Expressing, Cytokine Assay

Journal: The Scientific World Journal

Article Title: In Vivo Expression of Interleukin-37 Reduces Local and Systemic Inflammation in Concanavalin A-Induced Hepatitis

doi: 10.1100/2011/968479

Figure Lengend Snippet: Histological hepatitis score and IL-6 protein expression in the liver of mice after ConA-induced hepatitis. Areas of portal inflammation (a and b) and necrosis (c and d) in ConA-induced hepatitis in control mice (histology of IL-37-expressing mice not shown). Histological hepatitis score analyzed for IL-37 expressing mice (open bars) and control mice (closed bars) (e). IL-6 in liver lysate was analyzed by multiarray cytokine assay and is expressed as pg/mg total protein in liver lysate (f).

Article Snippet: Immunostaining against IL-37 in formalin-fixed liver tissue was performed with a polyclonal antibody against IL-37 (anti-human IL-1F7/FIL1 zeta goat IgG R&D-Systems) and a mouse monoclonal Ab [ ].

Techniques: Expressing, Cytokine Assay